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Riccardo Vicedomini
metaclade2
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df097cce
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df097cce
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May 08, 2020
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Riccardo Vicedomini
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@@ -40,30 +40,36 @@ where `[MetaCLADE2_DIR]` is MetaCLADE2 installation directory.
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@@ -40,30 +40,36 @@ where `[MetaCLADE2_DIR]` is MetaCLADE2 installation directory.
```
```
USAGE: metaclade2 -i <input_fasta> -N <name> [options]
USAGE: metaclade2 -i <input_fasta> -N <name> [options]
MANDATORY OPTIONS:
MANDATORY OPTIONS:
-i, --input <path> Input file of AA sequences in FASTA format
-i, --input <path> Input file of AA sequences in FASTA format
(protein sequences or predicted CDS)
-N, --name <str> Dataset/job name
-N, --name <str> Dataset/job name
MetaCLADE OPTIONS:
MetaCLADE OPTIONS:
-a, --arch Use DAMA to properly compute domain architectures
-a, --arch Use DAMA to properly compute domain architectures
(useful only for long protein sequences)
(useful only for long protein sequences)
-d, --domain-list <str> Comma-spearated list of Pfam accession numbers of
-d, --domain-list <str> Comma-spearated list of Pfam accession numbers of
the domains to be considered in the analysis
the domains to be considered in the analysis
(e.g., "PF00875,PF03441")
(e.g., "PF00875,PF03441")
-D, --domain-file <path> File that contains the Pfam accession numbers
-D, --domain-file <path> File that contains the Pfam accession numbers
of the domains to be considered (one per line)
of the domains to be considered (one per line)
-e, --evalue-cutoff <float> E-value cutoff
-W, --work-dir <path> Working directory, where jobs and results are saved
-E, --evalue-cutconf <float> Confidence threshold used by DAMA to add new domains into the architecture.
-W, --work-dir <path> Working directory, where jobs and results are saved
DAMA OPTIONS:
-e, --evalue-cutoff <float> E-value cutoff (default:1e-3)
OTHER OPTIONS:
-E, --evalue-cutconf <float> Confidence threshold to add new domains in the architecture.
(default:1e-10)
--overlappingAA <num> number (>=0) of amino acids allowed in the domain overlapping.
(default:30)
--overlappingMaxDomain <num> domain overlapping is allowed for at most <num>% of the match
(default:50)
OTHER OPTIONS:
-j, --jobs <num> Number of jobs to be created (default:16)
-j, --jobs <num> Number of jobs to be created (default:16)
-t, --threads <num> Number of threads for each job (default:1)
-t, --threads <num> Number of threads for each job (default:1)
-h, --help Print this help message
-h, --help Print this help message
-V, --version Print version
-V, --version Print version
SGE OPTIONS:
SGE OPTIONS:
--sge Run MetaCLADE jobs on a SGE HPC environment
--sge Run MetaCLADE jobs on a SGE HPC environment
--pe <name> Parallel environment to use (mandatory)
--pe <name> Parallel environment to use (mandatory)
--queue <name> Name of a specific queue where jobs are submitted
--queue <name> Name of a specific queue where jobs are submitted
...
...
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